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Secugen - DNA Sequencing - Analysis Offered

DNA Genotyping

Genotyping

By means of genotyping techniques, it is possible to visualize one or multiple amplified DNA fragments simultaneously, thus obtaining a pattern of specific bands for each species (interspecific variability) or that show variability among individuals from certain species (intraspecific variability).

  1. Fragments analyses (microsatellites)
  2. Other analyses: AFLPs, SNPs, SnapShot, BAC-fingerprinting...

Fluorophores used for labeling of the fragments

Dyes
Max A (nm)
Max E (nm)
Actual Intensity
6-FAM ™
494
522
100
JOE ™
528
554
100
HEX ™
535
553
50
VIC ®
538
554
100
NED ™
546
575
40
Cy3C
550
550
570
PET ®
558
595
25
ROX ™
587
607
12
LIZ ®
638
655
50

Matrix used for the combination of fluorophores selected by the customer

Matrix
Blue
Green
Yellow
Red
Orange
Utility
D
6-FAM™
HEX™
NED™ / CY3C
ROX™
----
Genotyping/Microsatellites
G5
6-FAM™
VIC ®
NED™
PET ®
LIZ ®
Genotyping/Microsatellites
E5
dR110
dR6G
dTAMRA™
dROX ™
LIZ ®
SNaPshot
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