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Sanger DNA Sequencing
Next Generation Sequencing
Cells Lines Identification
Fungi Bacteria Identification
PCR Amplicons Cloning
R & D
Secugen - Technical Solutions - Stuttering After Mononucleotide Stretches
Sequencing data quality is poor after stretches of 10 or more nucleotides of the same base due to mixed bases.
Polymerase slippage during DNA synthesis. This is a recognized limitation of the Sanger method.
Sequence from the reverse direction.
Use a poly-mononucleotide primer with a degenerate base (wobble) at the 3’ end.