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Secugen - Technical Solutions - Noisy Background

Sequence Appearance.

  • Background noise and odd peaks are present underneath the main sequence peaks, along with “N” dispersed in the sequence. Low raw signal.

Example.

Background

Noisy Background

Possibles Causes.

  • The most common cause for this is that the low signal above background is due to an insuficient template or primer input or an inefficient sequencing reaction for enzyme inhibition. A typical example is ethanol contamination in the template preparation.

Treatment.

  • Quantify properly template and primer; be sure you don´t have ethanol carry over from the purification columns.