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Secugen - Technical Solutions - Gradual Diminution of Signal

Sequence Appearance.

  • Sequencing signal gradually drops off.

Possibles Causes.

  • Excess DNA template or primer, disbalanced reaction.
  • GC or GT rich template (from bisulfide treated DNA, for example)


  • Carefully quantify your DNA template and primer prior to sequencing.
  • Sequence from the reverse direction.