Secugen - Gradual Diminution of Signal We use own and from thirds cookies to improve our services and your experience as user. If you continue using our website, we consider you accept their use. For more information visit our Cookies Policy. Close Secugen Español Customers Area Request For Quote Secugen Mision, Vision and Goals History Human Team Facilities Quality Policy DNA Sequencing Sanger DNA Sequencing Intro Analysis Offered Prices Service Request Next Generation Sequencing Analysis Offered GeneticDiagnostic Intro Analysis Offered Prices Genetic Counseling Analysis Request Other Services Cells Lines Identification Fungi Bacteria Identification PCR Amplicons Cloning R & D Projects Collaborators Contact Customer Support Contact us HHRR FAQs DNA Sequencing Genetic Diagnostic Secugen - Technical Solutions - Gradual Diminution of Signal Sequence Appearance. Sequencing signal gradually drops off. Possibles Causes. Excess DNA template or primer, disbalanced reaction. GC or GT rich template (from bisulfide treated DNA, for example) Treatment. Carefully quantify your DNA template and primer prior to sequencing. Sequence from the reverse direction.