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DNA Sequencing
Genetic Diagnostic
Secugen - Technical Solutions - Gradual Diminution of Signal
Sequence Appearance.
Sequencing signal gradually drops off.
Possibles Causes.
Excess DNA template or primer, disbalanced reaction.
GC or GT rich template (from bisulfide treated DNA, for example)
Treatment.
Carefully quantify your DNA template and primer prior to sequencing.
Sequence from the reverse direction.