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Mision, Vision and Goals
Sanger DNA Sequencing
Next Generation Sequencing
Cells Lines Identification
Fungi Bacteria Identification
PCR Amplicons Cloning
R & D
Secugen - Technical Solutions - Gradual Diminution of Signal
Sequencing signal gradually drops off.
Excess DNA template or primer, disbalanced reaction.
GC or GT rich template (from bisulfide treated DNA, for example)
Carefully quantify your DNA template and primer prior to sequencing.
Sequence from the reverse direction.